Pls1rom
Webb26 juni 2024 · Restriction sites of the multicloning sequence (MCS) of pLS1ROM and pLS1OM vectors are indicated. Unique restriction sites in pLS1ROM-GFP, pLS1ROM … Webbsequence of pLS1ROM plasmid (accession number: JN381945.1) [22]. Gene synthesis method was applied by synthesizing reverse complementary short DNA oligos using an automatic DNA synthesizer firstly, then short double-strand DNA fragments were obtained by anneal and joined by an one-step thermocycled in vitro recom-bination system [].
Pls1rom
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WebbLOCUS JN381945 6805 bp DNA circular SYN 25-JUL-2016 DEFINITION Cloning vector pLS1ROM plasmid pLS1ROM, complete sequence. ACCESSION JN381945 VERSION … WebbWe report the construction of a plasmid vector designed for regulated gene expression in Streptococcus pneumoniae. The new vector, pLS1ROM, is based on the replicon of the streptococcal promiscuous rolling circle replication (RCR) plasmid pMV158. We inserted the controllable promoter P M of the S. pneumoniae malMP operon, …
Webb5 dec. 2014 · S. pneumoniae R6 was grown in AGCH medium (Lacks, 1968) with 0.3 sucrose and 0.2% yeast extract at 37°C in static water bath, and supplemented with … Webbrecombinant plasmid pLS1ROM-GFP upon maltose induction. In contrast to the previously constructed pLS1RGFP plasmid, pLS1ROM-GFP exhibits structural and segregational …
Webb2.3.1. pLS1ROM-MCS derivatives . Seven putative pneumococcal TA genes from strain Hungary19A-6 (GI: 169832377) (Chan et al., 2012) were verified in the S. pneumoniae … Webb1 juni 1984 · The pneumococcal mal recombinant plasmid pLS70, which carries two strong promoters for transcription, could not be transferred and maintained intact i…
WebbWe report the construction of a plasmid vector designed for regulated gene expression in Streptococcus pneumoniae. The new vector, pLS1ROM, is based on the replicon of the streptococcal promiscuous rolling circle replication (RCR) plasmid pMV158. We inserted the controllable promoter P(M) of the S. pneumoniaemalMP operon, followed by a multi …
massage therapy school utahWebb17 sep. 2011 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our … hydraulic recoil pad mauser 98Webb149 was obtained in a previous work (Puga et al. 2024) using plasmid pLS1ROM–GFP 150 (Ruiz-Masó et al. 2012) for transformation. In this case, the transformed strain was 151 cultured on TSB plates supplemented with 5 µg/mL erythromycin from a stock culture 152 kept at –80 ºC in TSB supplemented with 15% (v/v) glycerol. 153 2.2. massage therapy school timeWebb1 juni 2003 · Plasmids pLS1ROM-GFP, pLS1ROM-Cherry, and pLS1OM-Cherry only harbor the pMV158 ssoA, which is fully functional in streptococci and is also recognized, … massage therapy school twin citiesWebbMICROBIOLOGY. ORIGINAL RESEARCH ARTICLE. published: 05 December 2014 doi: 10.3389/fmicb.2014.00677. Functional validation of putative toxin-antitoxin genes from the Gram-positive pathogen Streptococcus pneumoniae: phd-doc isthe fourth bona-fide operon massage therapy school virginia beachWebbMoreover, the PCN of constructs of the pLS1ROM/pLS1OM series, which were shown to have a slightly unstable inheritance (see section Segregational Stability of Different … massage therapy seattle premeraWebbMentioning: 18 - We report the construction of a plasmid vector designed for regulated gene expression in Streptococcus pneumoniae. The new vector, pLS1ROM, is based on the replicon of the streptococcal promiscuous rolling circle replication (RCR) plasmid pMV158. We inserted the controllable promoter P(M) of the S. pneumoniaemalMP operon, … massage therapy school tallahassee fl