2024 Drp1 phospho-ser616 antibody

Drp1 phospho-ser616 antibody

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August undefined, 2024

WebRabbit polyclonal antibody to DRP1 (phospho S616). A synthetic phosphorylated peptide around S616 of human DRP1 (NP_036192.2). Affinity purification. Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol. Shipped at 4°C. WebFigure 1: Anti-DRP1 (phospho Ser616) Antibody (AF8470) Western blot analysis of DRP1 (Phospho-Ser616) using UV treated 293 whole cell lysates. -/+ means absence or …

Phospho-DRP1 (Ser616) Antibody (PA5-64821)

WebTarget Information. A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian cells. It plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses ... WebPhospho-DRP1 (Ser616) Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser616. RRID: AB_2840952 Cite Format: Affinity Biosciences Cat# DF2972, RRID:AB_2840952. Conjugate: Unconjugated. Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho … cnrc royalmount

Mitochondrial fission requires DRP1 but not dynamins Nature

WebThe membrane was blocked with 10% skim milk at room temperature and incubated overnight at 4°C with the following primary antibodies: Drp1 (1:1000, ab184247, Abcam), phospho-Drp1 (Ser616 1:1000, 3455, Cell Signaling), Fis1 (1:1000, GTX111010, GeneTex), LC3B (1:2000, ab192890, Abcam) and VDAC (1:1000, GTX114187, GeneTex). WebPhospho-DRP1 (Ser616) Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser616. RRID: AB_2840952 Cite Format: Affinity Biosciences Cat# … WebFeb 6, 2024 · (4494) Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb Supplier Cell Signaling Technology. Host Rabbit Type Primary Clonality Recombinant (D9A1G4) Recombinant Yes Conjugate Unconjugated ... Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser616 of human … calcium channel blocker nursing consideration

Anti-DRP1 (Phospho-Ser616) Antibody (A51268) Antibodies.com

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WebImmunofluorescent analysis of Phospho-DNM1L (Ser637) in NCI-H1299 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min at 25°C) incubated with Phospho-DNM1L (Ser637) polyclonal antibody (Product # PA5-101038) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti … WebRabbit polyclonal antibody to DRP1 (phospho Ser637) for WB, IHC, ICC/IF and ELISA. 100% Guarantee. Price Match Guarantee. Product Size: 100µl. 200µl. Quantity: ... Rabbit Polycolonal antibody to DRP1 (Phospho-Ser616) for WB. A91223. Anti-DNM1L (phospho S637) Antibody. Rabbit polyclonal antibody to DNM1L (phospho S637) for WB. A12668. cnr collision repair in lafayette laWebSep 3, 2024 · WB was used to measure the expression levels of Drp1, Drp1 (Ser616), Drp1 (Ser637), and Fis1 at different time points (12–48 h). We found that Drp1 (Ser616) … cnr country

"WebDRP1 (Phospho-Ser616) Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser616. A synthesized peptide derived from human DRP1 (Phospho … " - Drp1 phospho-ser616 antibody

Drp1 phospho-ser616 antibody

Phospho-DRP1 (Ser616) Polyclonal Antibody

WebJun 2, 2024 · A–F. Decreased Drp1 S616 phosphorylation in PINK1-null HEK293 cells, MEF cells, and mouse substantial nigra tissues. Lysates of PINK1-null (PINK1 KO) and parkin … WebPhospho-DRP1 (Ser616) Antibody (BS-12702R) in IHC (P) Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen …

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WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH … WebNov 20, 2024 · As the phosphorylation of Drp1 at Ser616 was reported to be essential to its GTPase activity as well as mitochondrial fission [19], we further detected the level of phospho-Drp1 at Ser616 in the corneas after alkali burn by western blotting assay and immunofluorescence staining.As shown in Fig. 2 A and B, the phosphorylation level of …

WebPhospho-DRP1 (Ser616) Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser616. Species Reactivity: Human. Species predicted to react based on 100% sequence homology: The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested … WebThis antibody has been shown to work in applications such as: Precipitation, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and …

WebJan 14, 2024 · The mitochondrial network plays a critical role in the regulation of innate immune signaling and subsequent production of proinflammatory cytokines such as IFN-β and IL-1β. Dynamin-related protein 1 (DRP1) promotes mitochondrial fission and quality control to maintain cellular homeostasis during infection. However, mechanisms by which … WebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser616 of human DRP1. Antibodies are purified …

WebSpecificity: Phospho-DRP1 (Ser616) Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser616. Form: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage Stability: Store at -20 degree C. Stable for 12 months from date of receipt.

WebWestern blot - Anti-DRP1 (phospho S637) antibody (ab193216) All lanes : Anti-DRP1 (phospho S637) antibody (ab193216) at 1/500 dilution Lane 1 : Jurkat (Human T cell … cnrc sharepointWebPhospho-DRP1 (Ser616) Polyclonal Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser616. Visit thermofisher.com to view references and figures. … cnrc tlemcenWebApr 20, 2024 · b Western blot analysis for total Drp1, phospho-Drp1 Ser637 and phospho-Drp1 Ser616 in the retinas of AKAP1 −/ ... Primary antibodies included rabbit … cnrc royalmount adresseWebPhospho-DRP1 (Ser616) Polyclonal Antibody. PA5-64821 is a primary antibody from Invitrogen Antibodies. 16 Citations Host: Rabbit Clonality: Polyclonal Validations: Cell Treatment and Relative Expression. Applications: WB, IHC, and IF. Reactivity: Homo sapiens (Human), Mus musculus (House mouse), and Rattus norvegicus (Rat) cnrc uchicagoWebThe observed increase in the Drp1 gene expression was accompanied by an increase in the ratio of phospho-Drp1(Ser616) to total Drp1 (p = 0.0444; Figure 9d). Ser616 phosphorylation plays an important role in the regulation of Drp1 activity and promotes its translocation from the cytosol to the mitochondrial outer membrane and enables ... calcium channel blocker ointmentWebPhospho-DRP1 (Ser616) Polyclonal Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser616. 靶标信息 A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian … cnrc timed rightWebMay 27, 2024 · Antibodies against Phospho-RIP3 (Thr231/Ser232) and Phospho-Drp1 (Ser616) were purchased from Cell Signaling Technology (Danvers, MA, USA). The Aggresome Detection Kit was acquired from Abcam (Cambridge, MA, USA). cnrc toolbox