WebDec 12, 2011 · The % of NBD-PC reduced (P red) upon dithionite addition was calculated by normalizing the F0 to 1.0 and Ft as the fraction of F0. The activity of flippase in a proteoliposome preparation was taken as the difference between the percent reduced in the sample and the percent reduced in a liposome sample (control). Briefly, WebJul 13, 2024 · Notably, previous studies utilizing encapsulated water-soluble 2-NBD-glucose showed that dithionite cannot pass through the membrane of liposomes or proteoliposomes in conditions similar to ours 4 ...
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WebJun 1, 2003 · We observed dithionite quenching of fluorescence from the NBD-labeled probe molecule and measured the effect of DHA and other fatty acids on flip-flop. As a byproduct of these measurements we were also able to estimate relative permeability rates for bilayers to dithionite as a function of acyl chain double bond content. Webwhen dithionite is added to a sample, NBD should only be quenched in the outer leaet, diminishing uorescence to 45% of the initial intensity. This remaining intensity would Fig. 2 Dithionite quenching of NBD-PE LUV at various pH. a Chemical structure of NBD-PE. b Dithionite quenching of NBD-PE at pH 8.0 and 4.5. gu contingency\\u0027s
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WebSymmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50% decrease of its initial value corresponding to marker ... WebOct 8, 2016 · The decrease of NBD fluorescence occurring as the result of quenching of NBD by dithionite was monitored at the excitation of 463 nm and emission of 530 nm. The dithionite quenching led to the reduction of about 60–65% of NBD fluorescence signal, which corresponds to the NBD on the outer leaflet of the bilayer. WebOct 9, 1998 · Measurement of the extent of dithionite quenching of the fluorescence of 7-nitrobenz-2-oxa-1,3-diazol-4-yl- (NBD-) labeled lipids inserted into cellular and organellar membranes has been used to quantify their topological distribution and translocation. This assay provides a straightforward method for determining the fraction of NBD-lipid … boundary pillows