Ad293 cells
WebCulture media from Ad293 cells infected with AdM3 as well as plasma from mice infused with AdGFP and AdM3 were assessed from 4 to 12 weeks post adenoviral delivery following immunoprecipitation with anti-c-myc agarose beads. B. Chemokine activity was measured using the Boyden chamber migration assay. Calcein-AM labelled monocytes were … WebJan 6, 2024 · AD293 cells were seeded on a 6-well plate one day prior to transfection with the linearized rAdV14. The culture medium was replaced with a fresh preparation on days 3, 5, and 7, and GFP fluorescent images were taken 7 and 10 days post-transfection. The cytopathic effect caused by the adenovirus replication (bright field) and the expression of ...
Ad293 cells
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WebMar 8, 2024 · (A) Ad293 cells were transfected with pCMV-mCreER/pCMV-mTEVp. Inducers (1: R-T-, 2: R-T+, 3: R + T-, 4: R + T+) were added at 24 h after transfection. Cells were harvested at 24 h after induction and used for immunoprecipitation/western blot (IP/WB) analysis. (B) HSP90 was knocked down in Ad293 cells by siRNA. WebAD293 Cell 人胚肾细胞 BS-C00464971 HCC-1438细胞系;细胞生长特性:贴壁或悬浮,详见细胞说明书部分;细胞形态特性:详见细胞说明书;细胞传代方法:1:2-1:3传代;每周换液2-3次。 NCI-H2073细胞系;细胞生长特性
WebADI has always placed the highest emphasis on delivering products that meet the maximum levels of quality and reliability. We achieve this by incorporating quality and reliability … WebMar 4, 2024 · The day before transfection, AD293 cells were plated at a density of 1 × 10 4 cells per well in a white, 96-well tissue culture plate in DMEM containing 1% dialyzed FBS. AD293 cells were ...
WebJul 26, 2024 · AD293 cells were transfected with FLAG-p38α, FLAG-p38β, FLAG-p38γ or FLAG-p38δ expression plasmid and treated with DMSO (control) or 2 µM MG132 for 12 h. p38 expression and activation was detected by western blot analysis with FLAG and phospho-p38 (T180/Y182) antibody, respectively. β-actin was detected as a loading control. Web293AD Cell Line. Useful for both packaging and amplification of recombinant adenovirus. Derived from HEK 293 cell line. Selected for flattened morphology and firm attachment …
WebBioAssay record AID 1385827 submitted by ChEMBL: Selectivity ratio of IC50 for human GST-tagged PDE7A expressed in insect cells to IC50 for rhesus monkey brain PDE2A3 expressed in human AD293 cells.
Web人椎间盘髓核细胞 在低于正常生长温度情况下,会调整为静止期,或者慢速生长期,必须恢复 37 度的环境至少 3 个小时左右,才能重新调整到接近对数生长期的状态。. 人椎间盘髓核细胞产品说明: 名称:人椎间盘髓核细胞 货期: 1-2 周 代数: 2-3 代 规格: 7 0 %密度的25㎝2培养瓶的细胞一瓶 cisplatin ic50 sh-sy5yWebAD293 cells were transfected with plasmids expressing p38? mutants indicated. After 24 h, cells were treated with DMSO or 2 ?M MG132 for 14 h, and immunostained with SQSTM1 (green) and FLAG... cisplatin induced apoptosisWebStratagene's AD-293 cell line is a derivative of the commonly used HEK293 cell line, with improved cell adherence and plaque formation properties. HEK293 cells are human embryonic kidney cells transformed by sheared adenovirus type 5 DNA. 1 AD-293 cisplatin induced cardiotoxicityWeb293T CRL-3216 ™ 293T is an epithelial-like cell that was isolated from the kidney of a patient. This cell line was deposited by Stanford University and can be used in vaccine … cisplatin in animal beddingWebHowever, be sure to treat the cells gently to prevent damaging fragile cell membranes.) 3. Mix the cell suspension thoroughly and add to a suitable culture vessel. Gently rock or … cisplatin induced akiWebThe HEK 293 pattern most closely resembled that of adrenal cells, which have many neuronal properties. Given the location of the adrenal gland ( adrenal means "next to the … cisplatin induced hypocalcemiaWebJul 5, 2011 · AD293 cells were passaged and transfected with 200 ng of Halo-GR by Lipofectamine 2000 in 24-well plates. One day after transfection, cells were pulse-labeled with 20 nM TMR ligand for 15 min at 37°C according to the Promega manual, and then cells were washed with fresh phenol-free medium [plus 10% charcoal-stripped fetal bovine … cisplatin-induced aki